Trimerization and crystallization of reconstituted light-harvesting chlorophyll a/b complex.
نویسندگان
چکیده
منابع مشابه
Crystallization of the light-harvesting chlorophyll a/b complex within thylakoid membranes
We have found that treatment of the photosynthetic membranes of green plants, or thylakoids, with the nonionic detergent Triton X-114 at a 10:1 ratio has three effects: (a) photosystem I and coupling factor are solubilized, so that the membranes retain only photosystem II (PS II) and its associated light-harvesting apparatus (LHC-II); (b) LHC-II is crystallized, and so is removed from its norma...
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Diese Dissertation wurde selbstständig, ohne unerlaubte Hilfe erarbeitet. v Meinen Eltern Come, my friends, 'T is not too late to seek a newer world. Push off, and sitting well in order smite The sounding furrows; for my purpose holds To sail beyond the sunset, and the baths Of all the western stars, until I die. It may be that the gulfs will wash us down: It may be we shall touch the Happy Isl...
متن کاملChloramphenicol stimulation of light harvesting chlorophyll protein complex accumulation in a chlorophyll B deficient wheat mutant.
As compared with normal wheat leaves, the chlorina wheat mutant, designated CD3, has a high chlorophyll a/b ratio and a deficiency in the light harvesting chlorophyll protein (LHCP) complex. Applications of 200 micrograms per milliliter of d-threo-chloramphenicol to etiolated seedlings decreased the chlorophyll a/b ratio and increased the accumulation of the 27 kilodalton LHCP polypeptide and t...
متن کاملStark effect measurements on monomers and trimers of reconstituted light-harvesting complex II of plants.
The electric-field induced absorption changes (Stark effect) of reconstituted light-harvesting complex II (LHCII) in different oligomerisation states-monomers and trimers-with different xanthophyll content have been probed at 77 K. The Stark spectra of the reconstituted control samples, containing the xanthophylls lutein and neoxanthin, are very similar to previously reported spectra of native ...
متن کاملAssembly of the major light-harvesting chlorophyll-a/b complex: Thermodynamics and kinetics of neoxanthin binding.
The major light-harvesting chlorophyll-a/b complex in most higher plants contains three carotenoids, lutein, neoxanthin, and violaxanthin. How these pigments are assembled into the complex during its biogenesis is largely unknown. Here we show that neoxanthin but not lutein can dissociate from the fully assembled complex. Its equilibrium binding constant in a detergent system (0.1% n-dodecyl-be...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1994
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1994.tb06647.x